论文部分内容阅读
目的分析日本血吸虫尾蚴感染小鼠体内髓源抑制性细胞(myeloid-derived suppressor cells,MDSC)的富集情况,初步探索其在抗血吸虫感染免疫中的作用。方法日本血吸虫尾蚴用腹部贴片法感染C57BL/6小鼠(20条/鼠)24只。感染后1、2、6和8周采集小鼠(各6只)外周血,感染6、8周组小鼠脱颈处死后取脾组织,制备单细胞悬液。各时段同时设健康对照组(各6只)。通过流式细胞术检测小鼠脾组织和外周血中的Gr-1+细胞、CD11b+细胞及MDSC比例。通过CD4+T细胞增殖抑制试验验证感染小鼠Gr-1+粒细胞的功能。结果感染后6周和8周组小鼠外周血MDSC、Gr-1+细胞、CD11b+细胞分别约占总单核细胞(MNC)的38.2%~57.8%和47.1%~77.6%,28.9%~44.6%和40.4%~72.9%,36.0%~48.1%和40.3%~68.3%,显著高于健康对照组(15.1%~20.4%,8.4%~17.3%,9.8%~22.6%)、感染后1周(16.2%~19.8%,13.0%~16.8%,17.6%~19.4%)及2周组(19.8%~29.5%,17.2%~22.2%和20.9%~33.3%)(P<0.01)。而感染后1周、2周组与健康对照组相比,差异无统计学意义(P>0.05)。脾组织与外周血中的MDSC、Gr-1+细胞、CD11b+细胞变化趋势一致。此外,从感染小鼠脾组织分离到的Gr-1+细胞显著抑制刀豆球蛋白诱导的健康小鼠的CD4+T细胞增殖能力。结论日本血吸虫感染可诱导小鼠体内MDSC富集,且感染Gr-1+细胞可抑制正常CD4+T细胞增殖。
OBJECTIVE: To analyze the enrichment of myeloid-derived suppressor cells (MDSC) in mice infected with Schistosoma japonicum, and to explore its role in anti-schistosoma infection. Methods 24 C57BL / 6 mice (20 / mouse) were infected with cercariae of Schistosoma japonicum by abdominal patch method. Peripheral blood was collected at 1, 2, 6, and 8 weeks after infection, and the mice in 6 and 8 weeks after infection were sacrificed and their spleen tissues were removed to prepare single cell suspension. At each of the same time, a healthy control group (6 each). The proportion of Gr-1 + cells, CD11b + cells and MDSCs in spleen tissue and peripheral blood were detected by flow cytometry. The function of Gr-1 + granulocytes in infected mice was verified by CD4 + T cell proliferation inhibition assay. Results MDSC, Gr-1 + cells and CD11b + cells in peripheral blood of mice at 6 weeks and 8 weeks postinfection accounted for 38.2% -57.8% and 47.1% -77.6%, 28.9% -44.6% of total mononuclear cells (MNC) % And 40.4% -72.9%, 36.0% -48.1% and 40.3% -68.3%, respectively, which were significantly higher than those in healthy controls (15.1% -20.4%, 8.4% -17.3% and 9.8% -22.6% (16.2% -19.8%, 13.0% -16.8%, 17.6% -19.4%) and 2-week group (19.8% -29.5%, 17.2% -22.2% and 20.9% -33.3%, P <0.01). However, there was no significant difference between the 2 weeks and 1 week after infection (P> 0.05). The changes of MDSC, Gr-1 + cells and CD11b + cells in spleen and peripheral blood were consistent. In addition, Gr-1 + cells isolated from infected mouse spleen tissue significantly inhibited Concanavalin-induced CD4 + T cell proliferation in healthy mice. Conclusion Infection of Schistosoma japonicum can induce MDSC accumulation in mice, and infection of Gr-1 + cells can inhibit the proliferation of normal CD4 + T cells.