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目的探讨在金属钛表面类骨磷灰石上制备胶原蛋白与羟胆固醇复合生物活性涂层的可行性。方法研究于2013年12月在清华大学材料学院进行。采用酸-碱热处理结合仿生矿化沉积的方法在钛表面构建一层均匀的类骨磷灰石,采用物理吸附方法将胶原蛋白和羟胆固醇共同或单一固定在具有类骨磷灰石涂层的钛表面,构建钛表面的复合涂层。结果场发射扫描电镜和X射线衍射分析验证了钛表面的类骨磷灰石;衰减全反射-傅里叶变换红外光谱和X射线光电子能谱定性证明了胶原蛋白被成功固定在具有类骨磷灰石涂层的钛表面上;微二喹啉甲酸法和高效液相色谱法可测定固定在复合涂层上的胶原蛋白和羟胆固醇含量。结论通过酸-碱热处理和仿生矿化沉积方法可在钛表面构建一层均匀的类骨磷灰石,通过物理吸附方法可成功将胶原蛋白和羟胆固醇共同或单一固定在具有类骨磷灰石涂层的钛表面,构建钛表面的复合涂层。
Objective To explore the feasibility of preparing collagen and hydroxycholesterol bioactive coating on bone titanium apatite surface. The method study was conducted at Tsinghua University School of Materials Science and Technology in December 2013. A uniform layer of bone-like apatite was constructed on the surface of titanium by acid-base heat treatment combined with bionic mineralization. The collagen and hydroxycholesterol were immobilized on the surface of titanium with apatite-apatite coating by physical adsorption Titanium surface, the construction of titanium composite coating surface. Results The field emission scanning electron microscopy and X-ray diffraction analysis confirmed that the bone-like apatite on the titanium surface was characterized by attenuated total reflectance-Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Gray scale coated titanium surface; micro-bicinchoninic acid method and high performance liquid chromatography can be fixed in the composite coating on collagen and hydroxycholesterol content. Conclusions A uniform bone-like apatite can be constructed on the surface of titanium by acid-base heat treatment and biomimetic mineralization. Collagen and hydroxycholesterol can be successfully immobilized together with bone-like apatite The coated titanium surface builds a composite coating of titanium surface.