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为了定位控制白菜叶片紫色的pur基因,选用大白菜自交系09-680和紫色小白菜09N-742进行杂交构建了一个由307个单株组成的F2群体,采用群体分离分析法(Bulked segregant analysis,BSA)构建紫色和绿色池,对分布在白菜基因组10个连锁群上的125个InDel标记和100个SSR标记进行多态筛选,其中位于A3连锁群末端的2个In-Del标记BrID10999和BrID10399与紫色性状表现连锁。连锁分析发现,2个标记与pur基因的遗传距离分别为7.3,5.7 cM,位于pur基因的同侧。在此基础上,根据这些标记所在区域的BAC序列设计了23对SSR引物,其中来源于KBrH005P10的SSR标记BVRCP10-6位于pur基因的另一侧,距离pur基因仅1.9 cM。这些标记可有效用于白菜紫色性状的分子标记辅助育种,也为进一步精细定位和克隆pur基因奠定了基础。
In order to locate and control the purple pur gene in cabbage leaves, F2 population of 307 individuals was crossed with Chinese cabbage inbred line 09-680 and purple Chinese cabbage 09N-742, and the population segregant analysis (Bulked segregant analysis , BSA), 125 InDel markers and 100 SSR markers distributed on 10 linkage groups were polymorphic, including two In-Del markers BrID10999 and BrID10399 at the end of A3 linkage group Performance and purple chain. Linkage analysis revealed that the genetic distances between the two markers and the pur gene were 7.3 and 5.7 cM, respectively, located on the same side of the pur gene. Based on this, 23 pairs of SSR primers were designed based on the BAC sequences of the regions where these markers were located. Among them, the SSR marker BVRCP10-6 derived from KBrH005P10 was located on the other side of the pur gene, only 1.9 cM from the pur gene. These markers can be effectively used for molecular marker-assisted breeding of purple traits of cabbage and laid the foundation for further fine mapping and pur gene purifications.