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目的 建立高效液相色谱法(HPLC)同时测定大黄利胆胶囊中5种蒽醌类成分总含量的方法.方法 采用phenomenon luna C18色谱柱,流动相为甲醇-0.1%磷酸(82:18),检测波长254 nm,流速1.0 mL/min,柱温为30℃.结果 芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的线性范围分别为1.057~67.67 mg/L(r=0.9999),1.117~71.49 mg/L(r=0.9999),1.047~67.02 mg/L(r=0.9999),1.089~69.71 mg/L(r=0.9999),0.5968~38.20 mg/L(r=0.9996);平均加样回收率(n=6)分别为98.0%(RSD=1.69%),99.2%(RSD=2.19%),99.4%(RSD=1.89%),98.9%(RSD=0.76%),97.8%(RSD=1.26%).结论该方法简便,可行、重现性好,可作为大黄利胆胶囊的质量控制方法.“,”Objective To establish an HPLC method for determination of total content of five anthraquinones in Dahuang Lidan Capsules. Methods A phenomenon luna C18 column was used with the mobile phase consisted of methanol-0.1 % phosphoric acid solution (82:18) at a flow rate of 1.0 mL/min and the detection wavelength was set at 254 nm. The column temperature was maintained at 30 ℃. Results The linear ranges of aloe-emodin, rhein, emodin, chrysophanol, and physcion were 1.057-67.67 mg/L (r =0.9999), 1.117-71.49 mg/L (r=0.9999), 1.047-67.02 mg/L (r=0.9999), 1.089-69.71 mg/L (r=0.9999), 0.5968-38.20 mg/L (r=0.9996), respectively. The average recoveries (n=6) for the five ingredients were 98.0 % (RSD=1.69 %), 99.2 % (RSD=2.19 %), 99.4 % (RSD=1.89 %), 98.9 %(RSD=0.76 %), 97.8 % (RSD=1.26 %), respectively. Conclusion The method can be simple, feasible and reproducible, and can be used for the quality control of Dahuang Lidan Capsules.