目的　观察男性乙型肝炎表面抗原（ＨＢｓＡｇ） 阳性患者血清雌二醇（Ｅ２） 和睾酮（Ｔ） 水平的变化。方法　采用放射免疫分析法对１３５ 例男性ＨＢｓＡｇ 阳性患者（阳性组）和１０７ 例年龄匹配的男性ＨＢｓＡｇ 阴性者（ 阴性对照组）血清Ｅ２ 和Ｔ水平进行测量。结果　男性ＨＢｓＡｇ 阳性患者４ 个年龄组（２０～２９，～３９，～４９ ，～７０ 岁） 血清Ｅ２ 水平明显高于年龄匹配的阴性对照组（ Ｐ＜０－０１） ，而血清Ｔ水平明显低于年龄匹配的阴性对照组（ Ｐ＜ ０－０１） 。结论　男性ＨＢｓＡｇ 阳性患者血清Ｅ２ 水平增高，而血清Ｔ水平降低。 Objective To observe the changes of serum estradiol (E2) and testosterone (T) in male patients with positive HBsAg. Methods Serum E2 and T levels were measured by radioimmunoassay in 135 male HBsAg positive patients (positive group) and 107 age-matched male HBsAg negative patients (negative control group). Results The level of serum E2 in four age groups (20-29, ~ 39, ~ 49, ~ 70 years old) of male HBsAg positive patients was significantly higher than that of the age matched negative control group (P <0.01), while the serum T level was significantly lower Age-matched negative control group (P <0-01). Conclusions Serum E2 levels are elevated in male patients with HBsAg positive and serum T level is decreased.