论文部分内容阅读
目的研究食管癌组织中HSP70的表达情况,探索HSP70-多肽复合物分离的可行性途径。方法采用人HSP70基因探针P17与人食管癌组织中提取的RNA进行Northern杂交;用亲和柱层析法分离HSP70-多肽复合物。结果癌组织提取物出现了比正常组织较强的杂交信号,从食管癌组织中提取蛋白质,经一系列层析柱(Con-Asepharose、ADP-agarose、MonoQ及HSP70抗体亲和层析柱),分离到HSP70-多肽复合物。结论HSP70基因在食管癌组织中的高表达,为提取肿瘤HSP70-多肽复合物提供了可行性方法。
Objective To study the expression of HSP70 in esophageal cancer tissue and to explore the feasibility of HSP70-peptide complex separation. Methods Human HSP70 gene probe P17 was used for Northern hybridization with RNA extracted from human esophageal cancer tissue. HSP70-peptide complex was isolated by affinity column chromatography. Results A strong hybridization signal was found in the extracts of cancer tissues. The proteins were extracted from esophageal cancer tissues. After a series of chromatography (Con-Apharose, ADP-agarose, MonoQ and HSP70 antibody affinity chromatography) HSP70-polypeptide complex was isolated. Conclusion High expression of HSP70 gene in esophageal cancer tissue provides a feasible method for extracting tumor HSP70-polypeptide complex.