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目的探讨应用聚合酶链反应(PCR)检测慢性非细菌性前列腺炎(CPPS)患者前列腺液(EPS)中细菌基因,并与CPPS患者的临床治疗效果做相关性分析。方法以16SrRNA基因为靶序列,设计引物及寡核苷酸探针,采用PCR法检测标准菌株及135例CPPS患者前列腺液中细菌基因。所有CPPS患者均应用抗生素和中成药规范治疗,每2周为1疗程,每2周复查EPS常规1次,必要时适当调整药物。治疗3个月时,进行疗效评估。结果135例CPPS患者中16SrRNA检测结果阳性为78例,阳性率为57.78%。16SrRNA阳性组患者有效率84.6%,阴性组有效率52.6%;16SrRNA阳性组患者疗效明显优于阴性组。结论大部分CPPS患者的前列腺液中可以检测到细菌的16SrRNA基因,提示细菌感染在CPPS的发病中有重要作用。
Objective To investigate the use of polymerase chain reaction (PCR) in the detection of bacterial genes in prostatic fluid (EPS) of patients with chronic non-bacterial prostatitis (CPPS) and to analyze the clinical effect of CPPS. Methods 16S rRNA gene was used as target sequence to design primers and oligonucleotide probes. PCR was used to detect the bacterial genes in prostatic fluid from 135 standard CPPS patients. All patients with CPPS are treated with antibiotics and proprietary Chinese medicines, every 2 weeks for a course of treatment, review EPS regular every 2 weeks, if necessary, adjust the drug appropriately. Treatment for 3 months, the efficacy evaluation. Results The positive rate of 16S rRNA in 135 patients with CPPS was 78 and the positive rate was 57.78%. The positive rate of 16SrRNA positive group was 84.6%, while that of negative group was 52.6%. The effect of 16SrRNA positive group was better than that of negative group. Conclusion Bacterial 16S rRNA gene can be detected in prostatic fluid of most CPPS patients, suggesting that bacterial infection plays an important role in the pathogenesis of CPPS.