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目的研究夏枯草(Prunella vulgaris L.)果穗极性部位的化学成分。方法运用硅胶、ODS、Sephadex LH-20、MCI等色谱方法进行分离纯化,根据理化性质和波谱数据并参考文献鉴定化合物的结构。并通过MTT法,考查部分化合物抗乳腺癌细胞MCF-7、MDA-MB-231以及对正常乳腺细胞MCF-10A的活性进行筛选。结果从夏枯草果穗极性部位中分离得到12个化合物,分别鉴定为3,4,α-三羟基苯丙酸甲酯(1)、丹参素(2)、迷迭香酸甲酯(3)、3,4-二羟基苯甲酸(4)、槲皮素-3-O-葡萄糖苷(5)、金丝桃苷(6)、2a,3a,19a,24-四羟基-12-烯-28-熊果酸(7)、2a,3a,24-三羟基-12-烯-28-齐墩果酸(8)、胞苷(9)、胡萝卜苷(10)、(3S,5R,10S)-7-oxo-12-methoxyabieta-8,11,13-triene-3,11,14-triol(11)和2α,3α,24-三羟基-12,20(30)-二烯-28-齐墩果酸(12)。体外抗肿瘤活性筛选结果表明,化合物2、3、5、6对乳腺癌细胞MCF-7有明显抑制作用,化合物3对乳腺癌细胞MDA-MB-231有抑制作用,但都对正常乳腺细胞也有明显抑制作用。结论化合物9和11均为首次从该属植物中分离得到,体外MTT法活性筛选表明夏枯草极性部位化合物具有一定的抗乳腺癌细胞增殖作用。
Aim To study the chemical constituents in the polar parts of Prunella vulgaris L. Methods The compounds were separated and purified by chromatography on silica gel, ODS, Sephadex LH-20 and MCI. The structures of the compounds were identified according to their physico-chemical properties and spectral data and references. MTT assay was used to screen the activity of some compounds against breast cancer cells MCF-7, MDA-MB-231 and normal breast cells MCF-10A. Results Twelve compounds were isolated from the spikelets of Prunella vulgaris and identified as 3,4, α-trihydroxybenzenepropanoic acid methyl ester (1), danshensu (2), rosmarinic acid methyl ester (3) (4), quercetin -3-O-glucoside (5), hyperin (6), 2a, 3a, 19a, 24-tetrahydroxy-12-ene- 28-ursolic acid (7) 2a, 3a, 24-trihydroxy-12-ene-28- oleanolic acid (8) cytidine (9) ) -7-oxo-12-methoxyabieta-8,11,13-triene-3,11,14-triol (11) and 2a, 3a, 24-trihydroxy-12,20 (30) Oleanolic acid (12). In vitro antitumor activity screening results showed that compounds 2,3,5,6 significantly inhibited breast cancer cells MCF-7, Compound 3 on breast cancer cells MDA-MB-231 have an inhibitory effect, but both normal breast cells also have Obvious inhibitory effect. Conclusion Compounds 9 and 11 were isolated from this genus for the first time. The in vitro MTT activity screening showed that the compound of Prunella vulgaris polar region had a certain anti-proliferation effect on breast cancer cells.