论文部分内容阅读
目的探讨高糖及转化生长因子-β2(Transforming growth factor,TGF-β2)和高糖同时存在条件对体外培养的Müller细胞活力的影响,以及补肾活血中药的干预作用。方法以改良酶消化法体外培养大鼠Müller细胞,将P3细胞分别置于模拟正常、高糖、TGF-β2及TGF-β2和高糖同时存在条件下进行培养,并以补肾活血中药复方含药血清进行干预。实验分为正常对照组、TGF-β2组、高糖组、TGF-β2+高糖组、正常中药干预组、TGF-β2+高糖中药干预组;分别在试验干预12h、24h及48h后,以490型酶标仪测定各组细胞外液中乳酸脱氢酶(lactate dehydrogenase,LDH)的漏出量。结果 TGF-β2组和高糖组12h和24h的LDH漏出量与正常对照组比较均无明显差异(P>0.05),但48h的LDH漏出量均较正常对照组增多(P<0.05);TGF-β2组24h的LDH漏出量较高糖组增多(P<0.05);TGF-β2+高糖组24h和48h的LDH漏出量均较TGF-β2组减少(P<0.05);TGF-β2+高糖组48h的LDH漏出量较高糖组减少(P<0.05);补肾活血中药血清能降低24h时TGF-β2和高糖同时存在条件下LDH的漏出量,降低各时段正常条件下的LDH漏出量(P<0.05)。结论 Müller细胞对短期高糖状态以及浓度为150pg/ml的TGF-β2短期干预可能有一定的耐受性,但当高糖(50mol/l)以及TGF-β2(150pg/ml)干预48h后Müller细胞活力明显降低;补肾活血中药复方含药血清能提高正常及高糖和TGF-β2同时存在条件下视网膜Müller细胞膜的稳定性、增强Müller细胞活力可能是补肾活血中药复方防治糖尿病性视网膜病变的另一药物干预途径。
Objective To investigate the effects of high glucose and TGF-β2 and high glucose on the vitality of Müller cells in vitro and the intervention of Bushen Huoxue Chinese medicine. Methods Rat Müller cells were cultured in vitro by modified enzymatic digestion method. P3 cells were cultured in simulated normal, high glucose, TGF-β2, TGF-β2 and high glucose respectively. Serum for intervention. The experimental groups were divided into normal control group, TGF-β2 group, high glucose group, TGF-β2 + high glucose group, normal TCM intervention group and TGF-β2 + high glucose Chinese medicine intervention group. The amount of lactate dehydrogenase (LDH) in the extracellular fluid of each group was measured by microplate reader. Results Compared with the normal control group, there was no significant difference in LDH leakage between TGF-β2 group and high-glucose group at 12h and 24h (P> 0.05), but the leakage of LDH at 48h was higher than that of the normal control group (P <0.05) (P <0.05). The leakage of LDH at 24 h and 48 h in TGF-β2 + high glucose group was lower than that in TGF-β2 group (P <0.05) Compared with the control group, the release of LDH in group 48h decreased significantly (P <0.05). Bushenhuoxue serum could reduce the leakage of LDH in the presence of TGF-β2 and high glucose at 24h and reduce the leakage of LDH under normal conditions (P <0.05). Conclusion Müller cells may have some tolerance to short-term high glucose and short-term TGF-β2 at a concentration of 150 pg / ml. However, Müller cells may be resistant to short-term high glucose (50 pg / ml) and TGF- Cell viability was significantly reduced; Bushenhuoxu herbs containing serum can improve the normal and high glucose and TGF-β2 under the condition of simultaneous retinal Müller cell membrane stability and enhance the vitality of Müller cells may be Bushenhuoxue Chinese medicine compound to prevent and treat diabetic retinopathy A drug intervention route.