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目的:对小鼠精子进行氯化镉染毒10周,观察氯化镉对精子参数及精子DNA的影响。方法:以含不同浓度的氯化镉对小鼠进行染毒,分为阴性对照组、0.25%氯化镉组、0.5%氯化镉组和1.0%氯化镉组。染毒10周后,HE染色观察睾丸形态。通过附睾上游法收集精子,手工法检测精子的浓度、活力、活率,通过HE染色观察精子形态。通过精子染色质结构分析(SCSA)和彗星试验观察附睾精子损伤情况。结果:与阴性对照组相比,染毒组小鼠的睾丸形态无明显改变;小鼠附睾精子浓度、存活率均无统计学差异(P>0.05)。与阴性对照组相比,0.5%氯化镉组、1.0%氯化镉组小鼠附睾精子活力具有统计学差异(P<0.001);各染毒组小鼠附睾精子畸形率均有统计学差异(P<0.001)。附睾精子SCSA结果显示,0.5%氯化镉组、1.0%氯化镉组的精子DFI值升高(P<0.001)。彗星试验结果显示,各染毒组的精子彗尾长度、彗尾DNA%及彗尾位移长度均比阴性对照组增加(P<0.001)。结论:低剂量氯化镉染毒10周,不影响小鼠的睾丸形态,也不影响小鼠附睾精子浓度和存活率,但影响附睾精子活力和精子形态学,氯化镉浓度越高,附睾精子DNA损伤越大。
OBJECTIVE: To observe the effects of cadmium chloride on sperm parameters and sperm DNA in mouse spermatozoa for 10 weeks. Methods: The mice were exposed to cadmium chloride containing different concentrations and divided into negative control group, 0.25% cadmium chloride group, 0.5% cadmium chloride group and 1.0% cadmium chloride group. Ten weeks after exposure, HE staining was used to observe the morphology of testis. The sperm was collected by the epididymis upstream method and the sperm concentration, viability and viability were detected by manual method and the morphology of sperm was observed by HE staining. Epididymal sperm injury was observed by sperm chromatin structure analysis (SCSA) and comet assay. Results: Compared with the negative control group, there was no significant change in the testis morphology of the mice in the experimental group. There was no significant difference in the sperm concentration and survival rate between the mice and the control group (P> 0.05). Compared with the negative control group, the epididymal sperm motility of mice in 0.5% cadmium chloride group and 1.0% cadmium chloride group had statistical significance (P <0.001). The rates of epididymal sperm deformity in all exposure groups were significantly different (P <0.001). The epididymal sperm SCSA results showed that the DFI value of sperm in 0.5% cadmium chloride group and 1.0% cadmium chloride group increased (P <0.001). The results of comet assay showed that the sperm tail length, tail DNA length and tail length were higher than those in the negative control group (P <0.001). Conclusion: Low dose cadmium chloride exposure for 10 weeks does not affect the testicular morphology of mice, nor does it affect the sperm concentration and survival rate in mice, but affects the sperm motility and sperm morphology of epididymis. The higher concentration of cadmium chloride, Sperm DNA damage greater.