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“T”抗原(Thomselei-Friedenreich Antigen)被认为是癌相关抗元,包括大肠癌。为了澄清“T”抗原是否出现于大肠癌及其特点,本文比较了花生凝集素,多克隆抗“T”抗体以及单克隆抗“T”抗体在人类大肠各类组织。结果表明花生凝集素对大肠癌有较高的敏感性(91%),但在正常组织中特异性最差(68%)。单克隆抗“T”抗体的敏感性只有76%,但是100%特异性。多克隆抗“T”抗体的敏感性及特异性界于前两者之间。单克隆抗“T”抗体在腺瘤型息肉中出现率低于多克隆及花生凝集素,但与三种癌前病变指标有平行关系。在化生型息肉中花生凝集素的出现多于另两种抗体。各类细胞浆内多克隆及单克隆抗“T”抗体的出现率亦高于花生凝集素。大多数胚胎大肠组织都具有“T”抗原,这表明“T”抗原也是一种癌胚抗原。三种抗体所表现的不同,可能是由于其所识别结合的抗原不同所致。
The “T” antigen (Thomselei-Friedenreich Antigen) is considered to be a cancer-associated antigen, including colorectal cancer. In order to clarify whether “T” antigen appears in colorectal cancer and its characteristics, this paper compares peanut lectin, polyclonal anti-“T” antibodies, and monoclonal anti-“T” antibodies in human large intestine tissues. The results showed that peanut lectin was highly sensitive to colorectal cancer (91%), but was the worst specificity (68%) in normal tissues. The monoclonal anti-“T” antibody is only 76% sensitive but 100% specific. The sensitivity and specificity of the polyclonal anti-“T” antibody lies between the first two. The incidence of monoclonal anti-“T” antibodies in adenoma polyps was lower than that in polyclonal and peanut lectins, but parallel to the three precancerous lesions. Peanut agglutinin appeared in metaplastic polyps more than the other two antibodies. The frequency of polyclonal and monoclonal anti-“T” antibodies in all types of cytoplasm was also higher than that of peanut agglutinin. Most embryonic colon tissues have “T” antigens, indicating that the “T” antigen is also a carcinoembryonic antigen. The difference in the three antibodies may be due to the different antigens they recognize.