构建表达性载体和大鼠正常肝表达性cDNA文库,利用该库转染经致癌剂诱变的大鼠肝癌细胞CBRH-7919,得到和分离出一系列表型性状逆转的克隆。生长曲线、软琼脂测试和裸鼠实验表明这些整合了外源cDNA的克隆原有生长表型的恶性程度均有显著的降低。 An expression vector and rat normal liver expression cDNA library were constructed, and this library was used to transfect CBRH-7919, a rat hepatoma cell that was mutagenized by carcinogen, to obtain and isolate a series of clones whose phenotypic traits were reversed. Growth curves, soft agar assays, and nude mice experiments showed that the degree of malignancy of the original growth phenotypes of these clones incorporating the exogenous cDNA was significantly reduced.