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目的：研究化香树果序挥发油的化学成分及其体外抗肿瘤活性。方法：采用水蒸气蒸馏法从化香树果序中提取挥发油，通过气相色谱-质谱联用技术对其挥发油成分进行分析；采用MTT法观察其对人肝癌HepG2细胞、人鼻咽癌CNE-2细胞及人宫颈癌HeLa细胞的增殖抑制率及半数抑制浓度（IC50）。结果：从挥发油中共鉴定出28个化合物，占总离子流图峰面积的94.10%，其中含量较高的组分为（Z，Z，Z）-9，12，15-十八碳三烯酸（44.48%）、十六烷酸（23.30%）和斯巴醇（3.20%）等。化香树果序挥发油对人肝癌HepG2细胞、人鼻咽癌CNE-2细胞及人宫颈癌HeLa细胞均表现出不同程度的抑制活性，IC50值分别为848.554、451.835、301.253μg/mL。结论：化香树果序挥发油具有多种活性成分，对人肝癌HepG2细胞、人鼻咽癌CNE2细胞及人宫颈癌HeLa细胞均显示出了一定的抑制活性。“,”Objective: To analyze the chemical constituents of the essential oil from Platycarya strobilacea and investigate the antitumor activity in vitro. Methods: The essential oil of Platycarya strobilacea was extracted by steam distillation. The chem-ical components were separated and analyzed by GC-MS. The proliferations and half inhibition concentration (IC50) were ob-served by MTT assay in hepatocellular carcinoma cell HepG2,nasopharyngeal carcinoma cell CNE2 and cervical carcinoma cell HeLa, which treated with different concentrations of The essential oil of Platycarya strobilacea. Results: There were 28 com-pounds identified and accounted for 94.10% of the peak area from the total ion-current chromatogram. The higher content of components were (Z,Z,Z)-9, 12,15- calendic acid (44.48%), hexadecanoic acid (23.30%) and barbatol (3.20%), et al. The essen-tial oil showed different antitumor activities against the proliferation of HepG2,CNE2 and HeLa cells in concentration-dependent manner. The IC50 values of The essential oil of Platycarya strobilacea against HepG2, CNE2 and HeLa cells were 848.554、451.835、301.253 μg/mL respectively. Conclusion: The essential oil of Platycarya strobilacea contains various constituents which showed antitumor activities on HepG2, CNE2 and HeLa cells in vitro.