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本文报道了用化学发光分析法检测培养细胞中支原体的污染.实验以鼠脾细胞和人或牛的多形核白细胞做为指示细胞,基于污染了支原体的培养细胞能刺激依赖于氨基苯二酰肼的化学发光,用液体闪烁计数器测量化学发光(CL),判断支原体污染程度.本法适用于单层或悬浮细胞培养,具有敏感、快速的特点,可在3～5小时内观察结果.操作中没有放射性及其它有害化学物质,优于目前常用的DNA染色、电镜观察及琼脂板接种培养等方法.实验分三步进行:首先将人或牛静脉血,小鼠脾脏离心处理得到指示细胞,将指示细
In this paper, the chemiluminescence assay was used to detect the mycoplasma contamination in cultured cells.Experimental experiments using murine splenocytes and human or bovine polymorphonuclear leukocytes as indicator cells, based on mycoplasma-contaminated cells, Hydrazine chemiluminescence, chemiluminescence (CL) measurement with a liquid scintillation counter, to determine the degree of mycoplasma contamination.This method is suitable for single-layer or suspension cell culture, with sensitive, fast features, can be 3 to 5 hours to observe the results. In the absence of radioactive and other harmful chemicals, better than the currently used DNA staining, electron microscopy and agar plate inoculation and other methods.The experiment is divided into three steps: first human or cow venous blood, mouse spleen centrifuge to give instructions cells, Will indicate fine