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目的 :观察康胃方对沙土鼠幽门螺杆菌 (Hp)根除及胃粘膜细胞保护作用。方法 :蒙古沙土鼠 42只 ,随机分为病理模型组、三联治疗组及康胃方高、低剂量组 ,每日灌胃给予 Hp菌液 ,共 3次。用脲呼吸试验 (UBT)确认 Hp感染情况并接受相应的治疗药物。另 12只动物未予感染。治疗后第 4周处死动物 ,测定胃粘膜活性氧 (RO)、一氧化氮 (NO)、诱生型一氧化氮合成酶 (i NOS)、Mn SOD及 NPSH。结果 :Hp感染各组动物 ,UBT与胃粘膜所含 RO、NO、i NOS和 Mn SOD明显升高 ,但 NPSH显著下降 (P <0 .0 1)。经抗感染治疗后 UBT值降低 ,其他指标明显改善(P <0 .0 1)。结论 :康胃方对蒙古沙土鼠感染 Hp有明显的根除作用 ,并通过改善感染动物胃粘膜某些生化成分的含量或活性 ,发挥其细胞保护作用。
Objective: To observe the protective effect of Kang Wei Fang on eradication of Helicobacter pylori (Hp) and gastric mucosal cells in gerbils. METHODS: Forty-two Mongolian gerbils were randomly divided into pathological model group, triple therapy group, and Kangweifang high- and low-dose group. Hp broth was administered by gastric gavage three times a day. The urea breath test (UBT) was used to confirm the Hp infection and receive the appropriate therapeutic drug. Another 12 animals were not infected. Four weeks after treatment, the animals were sacrificed and the gastric mucosal reactive oxygen species (RO), nitric oxide (NO), inducible nitric oxide synthase (iNOS), Mn SOD, and NPSH were measured. RESULTS: In Hp-infected groups, the SOD, RO, NO, iNOS, and Mn SOD in UBT and gastric mucosa were significantly increased, but NPSH was significantly decreased (P < 0.01). UBT decreased after anti-infective treatment, and other indicators improved significantly (P < 0.01). Conclusion: Kang Weifang has a significant eradication effect on H. pylori infection in Mongolian gerbils, and exerts its cytoprotective effect by improving the content or activity of certain biochemical components in infected gastric mucosa.