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目的 通过对2016年北京市4例输入性黄热病病毒全基因组深度测序分析,以期对病毒进行溯源、分析疫苗有效性以及防控策略提供分子依据.方法 将荧光PCR检测为阳性的血液、尿液样本利用Ion Torrent PGM平台进行全基因组深度测序,通过MEGA 6.0软件中邻接法进行遗传进化分析,并采用Lasergene Protean软件进行E蛋白氨基酸变异分析及抗原性分析.结果 从首例病人的血液样本和其他3个病例的尿液样本中分别获得了黄热病病毒的全基因组序列.遗传进化树分析结果表明,4例黄热病病毒基因组与安哥拉71株(AY968064)高度同源,相似度分别达99.21％、98.11％、98.02％、98.39％.4例黄热病病毒E蛋白的氨基酸序列与安哥拉71株的序列分析相似度分别达99.6％、99.39％、99.01％、99.59％.4例黄热病病毒E蛋白抗原性与17D疫苗株(X03700)的E蛋白抗原性比较分析无明差异.结论 4例黄热病病毒都是安哥拉流行株类似株,目前使用的17D疫苗具有有效性.“,”Objective Four imported cases of yellow fever virus (YFV) in Beijing were identified by whole genome sequencing and presented for the genetic basis of the origin,vaccine effectiveness,prevention and control of the disease.Methods Blood sample (the first case),urine samples (other three cases) were positive for YFV by real-time PCR and were identified by Ion torrent PGM NGS system.A phylogenic tree was to construct using MEGA 6.0 software with 1000 bootstrap replications and antigenicity was analyzed by Lasergen Protean.Results The complete genome sequences of 4 YFV were obtained.The sequences of 4 cases were compared to Angola 71 strain (AY968064) with highly identity (99.21％,98.11％,98.02％,98.39％ respectively).There was no key amino acid mutations founded in the E protein domain of 4 YFV compared to that of Angola 71 strain with highly identity (99.6％,99.39％,99.01％,99.59％respectively).There was no key mutation for antigenic index of E protein in 4 YFVs compared to that of the YFV-17D vaccine (X03700).Conclusions The four YFVs were derived from the epidemic strains in Angola.Moreover,amino acid mutation in E protein of YFV has slightly influence on the effectiveness of the YFV-17D vaccine.