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目的探讨离心速度的不同对制备的富血小板纤维蛋白(platelet-rich fibrin,PRF)结构、白细胞与血小板回收率及生长因子释放量的影响。方法分别采用3种离心速度(2500rpm;3000rpm;4000rpm)制备PRF,使用组织学和扫描电镜观察PRF凝胶结构;血细胞分析仪检测白细胞、血小板浓度;酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测TGF-β1、PDGF-AB的浓度。结果 3种PRF凝胶的主体结构为纤维蛋白聚集形成的立体网状结构,血小板与白细胞主要集中在PRF凝胶的白膜层。2500rpm与3000rpm制备的PRF,血小板与白细胞回收率及TGF-β1、PDGF-AB的释放量高于4000rpm(P<0.05),2500rpm与3000rpm间的差异无统计学意义(P>0.05)。结论离心速度的不同会在一定程度上影响PRF的生物学特性,2500rpm与3000rpm是制备PRF较为理性的转速。
OBJECTIVE: To investigate the effects of different centrifugation speeds on the structure of platelet-rich fibrin (PRF), leukocyte and platelet recovery, and the release of growth factors. Methods The PRFs were prepared using three centrifugation speeds (2500rpm; 3000rpm; 4000rpm) respectively. The structure of PRF was observed by histology and scanning electron microscopy. The leukocyte and platelet concentrations were detected by hematology analyzer. The enzyme linked immunosorbent assay (ELISA) ) Was used to detect the concentration of TGF-β1 and PDGF-AB. Results The main structures of three kinds of PRF gel were three-dimensional network formed by fibrin aggregation. The platelets and leukocytes were mainly concentrated in the white coat of PRF gel. PRF, platelet and leukocyte recovery, release of TGF-β1 and PDGF-AB at 2500rpm and 3000rpm were higher than 4000rpm (P <0.05). There was no significant difference between 2500rpm and 3000rpm (P> 0.05). Conclusion The different centrifugal velocity will affect the biological characteristics of PRF to a certain extent. 2500rpm and 3000rpm are the rational speed of PRF.