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目的通过对入境集装箱中发现的死鼠进行实验室检测,加强口岸对入境啮齿动物携带鼠疫杆菌的监测力度。方法分别用胶体金和PCR方法对入境集装箱中发现的死鼠进行鼠疫杆菌检测。结果该样本鼠疫杆菌F1抗原阳性,pla基因和fra基因PCR扩增均为阳性,判定该样本为疑似鼠疫杆菌感染。结论在口岸一线加强对入境鼠类携带鼠疫杆菌检测具有重要意义。同时,应不断加强口岸公共卫生核心能力建设,加强公共卫生实验室建设及重大疫情检测技术储备。
OBJECTIVE To strengthen the monitoring of port-borne rodent-borne Yersinia pestis by laboratory testing of dead rats found in incoming containers. Methods The plague gold and PCR methods were used to detect the plague bacillus in the dead rats found in the entering containers. Results The samples were positive for the F1 antigen of plague, and both pla and fra genes were positive for PCR amplification. The samples were determined to be suspected Yersinia pestis infection. Conclusion It is of great importance to strengthen the front line of the port to carry the detection of Yersinia pestis in entry ports. At the same time, it is necessary to constantly strengthen the construction of the core competence of public health at ports and ports, strengthen the construction of public health laboratories and reserve technical reserves for major epidemics.