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通过Daul-PAM100叶绿素荧光-P700氧化还原动力学同步分析系统,研究冬季大叶黄杨(Euonmymus ja-ponicus)室内恢复过程,光系统Ⅱ(PSⅡ)和光系统Ⅰ(PSⅠ)电子传递动力学的转变机制。结果表明:冬季逆境条件下,遭受低温和强光双重胁迫的阳生叶PSⅡ光量子效率低于仅遭受低温胁迫的阴生叶,但阳生叶PSI量子效率Y(Ⅰ)高于阴生叶。室内恢复过程,阳生叶与阴生叶的OJIP荧光动力学和P700氧化还原动力学活性逐渐恢复,PSⅡ与PSⅠ恢复过程均分快速阶段和慢速阶段,但两个光系统并不同步,PSⅡ快速恢复阶段为0～43 h,PSⅠ为0～6 h,阳生叶室内恢复43 h,阴生叶室内恢复6 h后,Pm峰值出现时间由20 ms提前至2 ms。由此可见,冬季室内恢复过程中,大叶黄杨阳生叶与阴生叶的两个光系统间的电子传递发生了不同的转变。
Through the simultaneous analysis of chlorophyll fluorescence and P700 redox kinetics of Daul-PAM100, the mechanism of the indoor restoration of Euonymus japonicus (Euonymus japonica) in winter was studied, and the electron transfer kinetics of PSⅡ and PSⅠ were studied. . The results showed that under the conditions of winter stress, the photon efficiency of PS Ⅱ light exposed to low temperature and high light stress was lower than that of light shade only exposed to low temperature stress, but the quantum efficiency of PSI was higher than that of shade leaf. OJIP fluorescence kinetics and P700 redox kinetic activities recovered gradually in the process of indoor restoration. The recovery process of PSⅡ and PSⅠhad a rapid and slow phase, but the two light systems are not synchronized. PSⅡ The rapid recovery stage was 0-43 h, the PSⅠ was 0-6 h, the recovery was 43 h in the males, and the peak of the Pm was resumed from 20 ms to 2 ms after 6 h recovery in the shaded leaves. It can be seen from the above that in the winter indoor restoration process, the electron transfer between the two light systems of Euonymus japonicus and shade leaf has undergone different changes.