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从采自云南的滇重楼 (Parispolyphyllavar yunnanensis)根状茎韧皮部、木质部和种子中分离出 5 0株内生真菌 ,其中从根状茎的韧皮部中分离出内生真菌最多。建立了甾体类化合物的检测和分析方法。检测到 32株内生真菌的发酵培养物中含有甾体化合物 ,其中 5株内生真菌的发酵培养物中甾体化合物的产率超过了 5 0mg/L。
Fifty isolates of endophytic fungi were isolated from the rhizome phloem, xylem and seeds of Parispolyphyllavar yunnanensis collected from Yunnan. Among them, the most endophytic fungi were isolated from phloem of rhizomes. The establishment of steroidal compounds detection and analysis methods. Fermentation cultures of 32 endophytic fungi were detected to contain steroid compounds, of which steroid yield was over 50 mg / L in fermentation cultures of five endophytic fungi.