Software reliability was estimated based on NHPP software reliability growth models. Testing reliability and operational reliability may be essentially different. On the basis of analyzing similarities and differences of the testing phase and the operational phase, using the concept of operational reliability and the testing reliability, different forms of the comparison between the operational failure ratio and the predicted testing failure ratio were conducted, and the mathematical discussion and analysis were performed in detail. Finally, software optimal release was studied using software failure data. The results show that two kinds of conclusions can be derived by applying this method, one conclusion is to continue testing to meet the required reliability level of users, and the other is that testing stops when the required operational reliability is met, thus the testing cost can be reduced.