目的 :应用蛋白组学技术分析小儿哮喘缓解期患者血清蛋白,构建小儿哮喘缓解期肺脾气虚证候的辨证模型。方法 :采用弱阳离子磁珠结合表面加强激光解析电离飞行时间质谱技术对94例哮喘缓解期患儿和80例健康儿童的血清作蛋白组学检测,筛选小儿哮喘缓解期肺脾气虚证候的差异性蛋白峰,应用Biomarker Patterns软件建立小儿哮喘缓解期肺脾气虚证候的辨证模型。结果 :50例小儿哮喘缓解期肺脾气虚证患者和80例健康儿童血清作蛋白组学比较,有30个差异蛋白峰(P<0.05,倍数≥1.5),筛选4832.60、5917.16、6122.09、33314.46 m/z 4个差异蛋白峰构建的小儿哮喘缓解期肺脾气虚证候辨证模型,灵敏度为87.50%,特异度为90.00%;盲法检验灵敏度为85.70%,特异度为86.70%。小儿哮喘缓解期肺脾气虚证患者和小儿哮喘缓解期脾肾阳虚、肺肾阴虚证患者血清作蛋白组学比较,有22个差异蛋白峰(P<0.01)。结论 :采用WCX磁珠结合SELDI-TOF-MS技术可以检测小儿哮喘缓解期肺脾气虚证候的特异性血清蛋白峰,并建立灵敏度和特异度较高的小儿哮喘缓解期肺脾气虚证候的辨证模型。 OBJECTIVE: To analyze the serum protein in patients with remission stage of asthma by proteomics technology and to establish a syndrome differentiation model of lung, spleen and Qi deficiency syndrome in children with remission of asthma. Methods: The serum of 94 children with asthma remission and 80 healthy children were detected by proteomic method with weak cation magnetic beads combined with surface enhanced laser desorption / ionization time of flight mass spectrometry to screen the difference of syndromes of lung, spleen and qi deficiency in children with remission of asthma Sexual protein peak, using the Biomarker Patterns software to establish the syndrome differentiation syndrome of lung, spleen and qi deficiency in children with asthma during remission stage. Results: There were 30 differential protein peaks (P <0.05, multiple≥1.5) in serum of 50 children with pulmonary spleen-qi deficiency syndrome and 80 healthy children in remission stage. Screening 4832.60,5917.16,6122.09,33314.46 m / z four differential protein peaks in asthmatic children with asthma remission model syndrome differentiation of lung, spleen and qi deficiency syndrome, the sensitivity was 87.50%, specificity was 90.00%; blind test sensitivity was 85.70%, specificity was 86.70%. There were 22 differential protein peaks (p <0.01) in proteomics of patients with pulmonary spleen-qi deficiency syndrome and pediatric asthma remission with spleen-kidney-yang deficiency and lung-kidney-yin deficiency syndrome. CONCLUSIONS: The serum protein peaks of lung, spleen and qi deficiency syndromes can be detected by WCX magnetic beads combined with SELDI-TOF-MS technique and the establishment of the sensitivity and specificity of syndrome-specific syndromes Dialectical model.