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为了制备兔抗人血浆载脂蛋白ApoB-100的多克隆抗体,用超速离心法分离血浆载脂蛋白,经SDS-PAGE电泳检测后利用Sepharose 6B层析进行纯化得到ApoB-100蛋白,并用双向琼脂扩散试验鉴定。经紫外吸收法定量后免疫家兔,3次免疫后采血制备抗血清,抗血清经琼脂双扩散实验测定为阳性,效价达到1/64以上.
To prepare a polyclonal antibody to rabbit anti-human plasma apolipoprotein ApoB-100, plasma apolipoproteins were isolated by ultracentrifugation and purified by Sepharose 6B chromatography after SDS-PAGE electrophoresis to obtain ApoB-100 protein. Diffusion test identification. The rabbits were immunized by UV absorbance method and the antiserum was collected after 3 immunizations. The antiserum was positive by agar double diffusion assay, and the titer reached 1/64 or above.