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目的 :观察腺相关病毒 (adeno -assoiatedvirus,简称AAV)载体介导的人β2 肾上腺素能受体 (简称β2 -AR)基因和增强型绿色荧光蛋白基因 (简称EGFP)对心肌细胞的转染及其表达。方法 :构建以串联方式携带 β2 -AR、EGFP基因的AAV载体 ,转染
OBJECTIVE: To observe the effect of AAV vector-mediated transfection of human β2-adrenoceptor (EGFP) and enhanced green fluorescent protein (EGFP) on cardiomyocytes and to investigate the effect of adenovirus vector- Its expression. Methods: AAV vector carrying β2-AR and EGFP genes in series was constructed and transfected