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目的寻找抑制视网膜、玻璃体内细胞增生的有效药物；明确抗增生药物秋水仙碱、道诺霉素和５－氟尿嘧啶（５－Ｆｕ）对体外培养的人视网膜胶质（ｒｅｔｉｎａｌ ｇｌｉａ，ＲＧ）细胞的作用。方法用 ＭＴＴ法测定秋水仙碱（０．５～１６．０μｇ／ｍｌ）、道诺霉素（０．１～３．２μｇ／ｍｌ）和５－Ｆｕ（０．５～１６．０μｇ／ｍｌ）对体外培养人ＲＧ细胞的作用。结果秋水仙碱（１．０～１６．０μｇ／ｍｌ）、道诺霉素（０．２～３．２０μｇ／ｍｌ）和 ５－Ｆｕ（１．０～１６．０μｇ／ｍｌ）３组药物对培养细胞均有抑制作用，与对照组均差别显著（Ｐ＜０．０１），ＩＤ_（５０）分别为３．１１μｇ／ｍｌ，０．７９μｇ／ｍｌ和５．２３μｇ／ｍｌ。结论秋水仙碱、道诺霉素和５－Ｆｕ对ＲＧ细胞有明显抑制作用。
OBJECTIVE To find effective drugs for inhibiting the proliferation of retinal and intravitreal cells. To investigate the effects of colchicine, daunomycin and 5-Fu on the proliferation of human retinal glia (RG) cells cultured in vitro effect. Methods Colchicine (0.5-16.0μg / ml), daunomycin (0.1-3.2μg / ml) and 5-Fu (0.5-16.0μg / ml) On the role of human RG cells cultured in vitro. Results Colchicine (1.0-16.0 μg / ml), daunomycin (0.2-3.20 μg / ml) and 5-Fu (1.0-16.0 μg / ml) (P <0.01). The ID50 values were 3.11μg / ml, 0.79μg / ml and 5.23μg / ml, respectively. Conclusion Colchicine, daunomycin and 5-Fu have significant inhibitory effect on RG cells.