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目的：探讨自制的含组氨酸、Ｂｉｍａｋａｌｉｍ的细胞内液型胶体液（ＨＢＳ）对离体心脏的保存作用。方法：健康雄性ＳＤ大鼠１６只，体重２４０～２６０ｇ。随机均分为两组，采用微量灌注技术，分别用ＨＢＳ和托马斯停搏液（ＳＴＳ）４℃保存离体鼠心２４小时，再灌注６０分钟进行比较研究。结果：ＨＢＳ组心功能恢复程度明显优于ＳＴＳ组，再灌注期ＬＤＨ、ＥＴ释放量ＨＢＳ组低于ＳＴＳ组，ＨＢＳ组心肌ＡＴＰ含量高于ＳＴＳ组两倍以上，心肌超微结构保持完好。结论：ＨＢＳ低温保存鼠心２４小时效果明显优于临床上常用的ＳＴＳ。
OBJECTIVE: To investigate the preservation effect of self-made intracellular fluid colloidal fluid (His) containing histidine and Bimakalim on isolated heart. Methods: Sixteen healthy male SD rats weighing 240-260g. The rats were randomly divided into two groups. Microvessel perfusion technique was used to save isolated rat heart for 24 hours at 4 ℃ with HBS and STS respectively. The rats were reperfused for 60 minutes. Results: The recovery of cardiac function in HBS group was significantly better than that in STS group. The LDH and ET release in HBS group were lower than those in STS group during reperfusion. The myocardial ATP content in HBS group was more than twice that in STS group, and the myocardial ultrastructure remained intact. Conclusion: HBS preservation rat heart at 24 hours was significantly better than the clinically used STS.