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本研究以普通小麦(TriticumaestivumL.;2n=6x=42,AABBDD)为母本,以东方旱麦草(Eremopyrumorientale(L.)Jaub.etSpach;2n=4x=28)为父本,首次成功地获得了属间远缘杂种F1,其平均结实率为0.08%。利用植物细胞工程技术,对杂种幼胚愈伤组织的诱导、胚性无性系的建立、植株再生、壮苗培养等,最终获得了生长正常的杂种F1植株。同时,通过对杂种幼胚愈伤组织、根尖细胞的细胞学观察,结果表明该杂种为真杂种,即2n=5x=35(预期染色体数)的杂种细胞占主体;另外,因组培过程中发生了染色体数目的变异,故也有少量2n=28-34染色体数的细胞。以上杂种的获得为将旱麦草优异基因向小麦的转移奠定了基础。
In this study, the male parent of Triticum aestivum L. (2n = 6x = 42, AABBDD) and the male parent of Eremopyrumorientale (L.) Jaub. Et Spach; 2n = 4x = Intergeneric distant hybrid F1, the average seed set rate of 0.08%. Plant cell engineering was used to induce the hybrid immature embryo callus, establishment of embryogenic clones, plant regeneration and strong seedling culture. Finally, the hybrid F1 plants with normal growth were obtained. At the same time, the hybridization of immature embryos callus and root tip cells cytological observation showed that the hybrids are true hybrids, that 2n = 5x = 35 (expected number of chromosomes) accounted for the main hybrid cells; In addition, due to tissue culture process Occurred in the chromosome number of variations, it is also a small amount of 2n = 28-34 chromosome number of cells. The accession of the above hybrid laid the foundation for the transfer of excellent genes of aescula propolis to wheat.