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取猪卵巢，从２～６ｍｍ有腔卵泡中抽取未成熟的卵丘卵母细胞复合体。分组处理后，各取１０枚按常规方法制片，光镜和电镜下观察测量。结果表明，对照组的卵丘细胞呈多层包围卵母细胞，其胞质突起与卵母细胞微绒毛交叉伸入透明带中，卵周隙尚未出现。高尔基复合体在卵皮质区成团分布。皮质颗粒位置分散。线粒体多成群存在于卵皮质区，嵴少，基质密度高。脂滴遍布胞质，由间断的滑面内质网包统。培养成熟后，卵丘细胞扩展松散，其突起和卵母细胞微绒毛，均自透明带中缩回，卵周隙出现。皮质颗粒成排贴于质膜下。高尔基复合体未见或仅残留少量囊泡。线粒体中出现空泡。脂滴内絮状物增多。卵母细胞生发泡破裂，第一极体分出，核处于第２次成熟分裂中期。受精后皮质颗粒内容物外倾或颗粒完整外排，线粒体多呈圆形。雌、雄原核形成，致密核仁出现。结果说明，体外受精与体内受精过程基本相同，仅有某些环节不完整或不规则。
Pig ovary, from 2 ~ 6mm antral follicles extracted immature cumulus oocyte complexes. After grouping, 10 samples were taken according to the conventional method and observed under light microscope and electron microscope. The results showed that the control group of cumulus cells surrounded by multiple layers of oocytes, cytoplasmic protrusions and oocyte microvilli cross into the zona pellucida, the perivitelline has not yet appeared. Golgi complex in the cortex distribution of clumps. Cortical particle size distribution. Mitochondria in the coccyx population, less crest, high matrix density. Lipid droplets in the cytoplasm, interrupted by the smooth endoplasmic reticulum envelop. After maturation, the cumulus cells are loosely expanded, their protrusions and oocyte microvilli are both retracted from the zona pellucida and the perivitelline space appears. Cortical particles in a row under the plasma membrane. No or only small amount of vesicles remained in the Golgi complex. Cavitation in mitochondria. Lipid droplets within the increased. Ovarian germinal vesicle rupture, the first polar body separation, the nucleus in the second mature metaphase. After fertilization, the content of cortical granule camber or complete efflux of granules, mostly round mitochondria. Female, male original nuclear formation, dense nucleolus appear. The results show that in vitro fertilization and in vivo fertilization process is basically the same, only some of the links are incomplete or irregular.