论文部分内容阅读
目的 比较2种制备急性痛风性关节炎小鼠模型的方法.方法 将18只C57BL/6♂小鼠随机分3组, 分别采用踝关节腔和足垫注射50 mg·mL-1尿酸钠混悬液25μL的造模法复制急性痛风性关节炎模型, 空白组注射0.9％氯化钠注射液.比较3组小鼠体质量、足肿胀度, ELISA测定踝关节液和血清中的IL-1β表达, 并进行组织形态学观察.结果 各组造模前后体质量无统计学差异;踝关节组关节液中IL-1β表达最高, 与足垫组和空白组比较具有统计学意义 (P空白组.结论 采用踝关节造模法, 取踝关节液作为ELISA样本, 有利于急性痛风性关节炎的动物实验研究.“,”OBJECTIVE To compare two methods for preparing mouse models of acute gouty arthritis. METHODS Eighteen C57 BL/6 male mice were divided into 3 groups randomly, building acute gouty arthritis model mice with 25 μL 50mg·mL-1 monosodium urate injection into ankle joint cavity and toe pad respectively, blank group using 0.9％ NaCl instead.Weight and foot swelling were compared, as well as expression of IL-1β in ankle fluid and serum. Furthermore, observed the histomorphology in each group. RESULTS There was no statistical difference in weight before or after modeling. The highest expression of IL-1β was found in the articular fluid of the ankle joint group, which was statically significant compared with the foot pad group and blank group (P blank group. CONCLUSION Using ankle joint modeling method and taking ankle joint fluid as ELISA sample is conducive to the animal experimental study of acute gouty arthritis.