【摘 要】
：
目的 观察移植过表达缝隙连接蛋白43(Cx43)的骨髓间充质干细胞(BMSC)对心肌梗死后心力衰竭大鼠的治疗作用.方法 将60只SD大鼠分为4组,每组15只.假手术组;DMEM/F12组:注射DMEM
【机 构】
：
华中科技大学同济医学院附属协和医院心内科
【基金项目】
：
湖北省生物靶向治疗研究重点实验室开放基金;
论文部分内容阅读
目的 观察移植过表达缝隙连接蛋白43(Cx43)的骨髓间充质干细胞(BMSC)对心肌梗死后心力衰竭大鼠的治疗作用.方法 将60只SD大鼠分为4组,每组15只.假手术组;DMEM/F12组:注射DMEM/F12培养基;EGFP(增强的绿荧光蛋白)组:移植转染EGFP 基因的BMSC;Cx43组:移植转染Cx43基因的BMSC.采用结扎前降支的方法,建立心肌梗死模型,于梗死后30 min,行细胞移植.移植后4周,超声心动图检测心脏功能并取心脏组织.Masson染色检测梗死面积及胶原纤维含量.激光共聚焦显微镜观察EGFP基因及Cx43的表达.RT-PCR检测移植区心肌组织Cx43 mRNA含量.结果 EGFP组和Cx43组心功能明显改善[左室射血分数分别为(76.0±6.9)%、(82.9±5.0)%,P＜0.05],心肌梗死面积缩小,胶原纤维含量降低[分别为(13.6±4.7)%、(15.9±3.2)%,P＞0.05],Cx43组心脏重构指标改善更明显.激光共聚焦显示EGFP组和Cx43组均有BMSC存活,并与宿主心肌组织间形成缝隙连接.DMEM/F12 组Cx43 mRNA水平较假手术组显著降低(0.18±0.05比0.78±0.14,P0.05).Cx43组Cx43 mRNA水平较DMEM/F12 组及EGFP组明显增加(0.39±0.14 比 0.18±0.05,P
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