正交型发光二极管诱导荧光检测器的研制

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以发光二极管为激发光源,以多模光纤代替透镜组作为激发光路,利用毛细管作为检测池,研制了一台小型、低功耗的荧光检测器。采用正交型光路设计,借助高精度机械加工的自校准平台,使光纤-毛细管-荧光收集透镜实现自校准。采用流动注射模式,以荧光素钠为标准样品对该检测器性能进行了测试,最低检测限为6.75×10-8 mol· L-1(S/N =3),在1.35×10-7~43.2×10-7 mol·L-1范围内线性关系良好(R 2=0.9992),稳定性良好(RSD<1%)。结合高效液相色谱系统,分离了异硫氰酸荧光素标记的4种氨基酸。
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