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目的为了研究肿瘤坏死因子（ＴＮＦ）的抗肿瘤机制，观察了ＴＮＦ诱导ＨＬ－６０细胞凋亡的作用。方法用流式细胞分析及ＤＮＡ电泳分析检测ＨＬ－６０细胞发生凋亡的情况。结果ＴＮＦ（４０μｇ／Ｌ）处理细胞后，流式细胞仪分别在６０ｈ，８４ｈ出现凋亡特有的ＡＰ峰（分别占６３．５％、８５．６％）；琼脂糖凝胶电泳显现出特征性的ＤＮＡ“梯状”带。结论ＴＮＦ（４０μｇ／Ｌ）具有诱导ＨＬ－６０细胞凋亡的作用。推测ＴＮＦ的抗肿瘤作用可能与诱导细胞凋亡有关
Objective To investigate the antitumor mechanism of tumor necrosis factor (TNF) and observe the effect of TNF on the apoptosis of HL-60 cells. Methods The apoptosis of HL-60 cells was detected by flow cytometry and DNA electrophoresis. Results After treatment with TNF (40μg/L), apoptosis-specific AP peaks (63.5% and 85.6%, respectively) appeared at 60h and 84h after flow cytometry, respectively; agarose gel electrophoresis showed characteristic characteristics. The “ladder” of DNA. Conclusion TNF (40μg/L) can induce apoptosis in HL-60 cells. Presumably the antitumor effect of TNF may be related to the induction of apoptosis