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目的探讨Clusterin蛋白在男性不育症患者睾丸组织中的表达及意义。方法采用免疫组化染色法和Western blot法检测了10例正常睾丸组织,8例梗阻性不育患者睾丸组织,13例非梗阻性不育患者睾丸组织标本中Clusterin蛋白的表达水平。结果免疫组化染色结果表明,3组睾丸组织中C1usterin蛋白都有表达,正常睾丸组织、梗阻性不育患者睾丸组织、非梗阻性不育患者睾丸组织中阳性或强阳性表达率分别为100%(10/10),87.5%(7/8),46%(6/13),非梗阻性不育睾丸组织中Clusterin蛋白表达水平显著低于正常睾丸组织(P<0.05)。Western blot法检测结果也表明,该蛋白在非梗阻性不育睾丸组织中的表达较正常睾丸组织显著降低,而在梗阻性不育睾丸组织中的表达无显著性降低。结论 Clusterin蛋白的表达降低与非梗阻性不育症的发生密切相关。
Objective To investigate the expression and significance of Clusterin in testicular tissue of male infertility patients. Methods Immunohistochemical staining and Western blot were used to detect the expression of Clusterin protein in 10 normal testis tissues, 8 testicular tissues of obstructive infertility patients and 13 non-obstructive infertility patients. Results Immunohistochemical staining showed that C1usterin protein was expressed in testis tissue of all three groups. The positive or strong positive rates of testicular tissue in testis tissue and non-obstructive infertility patients in normal testicular tissue and obstructive infertility patients were 100% (10/10), 87.5% (7/8) and 46% (6/13) respectively. The expression of Clusterin in non-obstructive testicular tissue was significantly lower than that in normal testicular tissue (P <0.05). The result of Western blot also showed that the protein expression in non-obstructive testicular tissue was significantly lower than that in normal testicular tissue, but not in obstructive sterile testis tissue. Conclusions The decreased expression of Clusterin protein is closely related to the occurrence of non-obstructive infertility.