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用底物尿酸的紫外吸收变化跟踪反应,用单或双底物米氏方程和初速度估算苛求芽孢杆菌胞内尿酸酶最大反应速度,按Arrenius经验公式得到该尿酸酶在pH 9.2和pH 7.4的活化能,但这些活化不能解释其在两个pH下的催化活性差异.该酶同常见嘌呤衍生物氧嗪酸、黄嘌呤和尿酸有亲和力,但对尿酸亲和力最低,且亲和力与嘌呤衍生物解离成阴离子的能力相关.在生理条件下,该酶对尿酸的亲和力相对于最适条件有显著下降.据此推测,优化静电相互作用选择性增强该酶对尿酸的亲和力是提高其成药性的可能措施之一.
The uric acid substrate UV-absorbance change tracking reaction, single or double substrate Mie equation and initial velocity estimation of Bacillus cereus uricase maximum reaction rate, according to Arrenius empirical formula obtained uricase at pH 9.2 and pH 7.4 Activation energy, but these activation can not explain its difference in catalytic activity at two pHs.The enzyme with the common purine derivatives otoxazine acid, xanthine and uric acid affinity, but the lowest affinity for uric acid, and affinity and purine derivative solution Which is related to the ability to form anions.Under physiological conditions, the affinity of the enzyme for uric acid decreased significantly relative to the optimal conditions.According to the above, it is presumed that the optimization of the electrostatic interaction to selectively enhance the affinity of the enzyme for uric acid is to improve its drug- One of the possible measures.