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rbcL是编码光合关键酶1,5-二磷酸核酮糖羧化酶(Rubisco)大亚基的基因。运用mRNA差异显示技术(DDRT-PCR)并通过5′RACE(rapidamplificationofcDNAends)从高原植物川草2号老芒麦(ElymussibiricusL.cv.‘chuancaoNo.2’)中获得了受增强UV-B辐射抑制的rbcL基因,该基因全长cDNA为1.51kb,开放阅读框(ORF)长1.434kb,编码477个氨基酸。氨基酸序列与Elymustrachycaulus中的Rubisco大亚基具有97%的同源性、与Triticumaestivum和Hordeumcomosum的Rubisco大亚基同源性均为98%。Northern杂交分析表明,增强UV-B辐射后6h,rbcL基因表达受到强烈抑制,处理后60h,其表达几乎完全被抑制,表明即使是长期生长在高原地区、强UV-B辐射条件下的高原物种,在受到较强的UV-B辐射后,其rbcL基因的转录也会受到抑制。
rbcL is a gene encoding the Rubisco large subunit, a photosynthetic key enzyme. Inhibition of enhanced UV-B radiation was obtained from the plateau plant Elymus biricus L.cv.’chuancao No. 2 ’using mRNA differential display technology (DDRT-PCR) and by 5’RACE (Rapid Amplification of cDNAs) The full-length cDNA of rbcL gene was 1.51kb in length and 1.434kb in open reading frame (ORF) encoding 477 amino acids. The amino acid sequence has 97% homology with Rubisco large subunit in Elymustrachycaulus and 98% homology with Rubisco large subunit of Triticum aestivum and Hordeumcomosum. Northern blot analysis showed that the expression of rbcL gene was strongly inhibited 6h after enhanced UV-B radiation, and its expression was almost completely inhibited 60h after treatment, indicating that even long-term growth in the plateau region, high UV-B radiation of plateau species , Under the strong UV-B radiation, its rbcL gene transcription will be inhibited.