目的：研究小檗碱（Ｂｅｒ）对心室肌细胞钙通道的影响．方法：全细胞膜片箝技术．结果：Ｂｅｒ（１０，３０μｍｏｌ·Ｌ－１）使豚鼠心室肌细胞Ｌ型钙流由１４００±２４７ｐＡ分别减至９７８±２０４ｐＡ及６１７±２３ｐＡ（ｎ＝５，Ｐ＜００５），抑制效应呈浓度依赖及非频率依赖，其电流－电压曲线的峰值下降．Ｂｅｒ（１０μｍｏｌ·Ｌ－１）使Ｌ型钙流失活曲线的最大半激活电压由－２７８ｍＶ变为－３４２ｍＶ，斜率因子由９２２变为１３０３，对激活曲线无影响．Ｂｅｒ（１０，３０μｍｏｌ·Ｌ－１）使Ｔ型钙流峰值由加药前的１５４±８０ｐＡ降至１０１±７８ｐＡ及４８±４５ｐＡ（ｎ＝８，Ｐ＜００５）．结论：Ｂｅｒ对Ｌ和Ｔ型钙通道均有抑制作用． Objective: To study the effect of berberine on calcium channel in ventricular myocytes. Methods: Whole cell patch clamp technique. Results: Ber (10,30μmol·L-1) decreased the L-type calcium flux in guinea pig ventricular myocytes from 1400 ± 247pA to 978 ± 204pA and 617 ± 23pA (n = 5, P <005) In a concentration-dependent and frequency-dependent manner, the peak value of the current-voltage curve decreases. Ber (10μmol·L-1) changed the maximum half-activation voltage of L-type calcium loss-deactivation curve from -278mV to -342mV and the slope factor from 922 to 1303, which had no effect on the activation curve . Ber (10,30μmol·L-1) decreased the peak value of T-type calcium flow from 154 ± 80pA before administration to 101 ± 78pA and 48 ± 45pA (n = 8, P <005). Conclusion: Ber has inhibitory effects on L-type and T-type calcium channels.