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为观察中药双黄连对人胚脑柯萨奇B_3病毒(CVB_3)脑炎模型的病变影响,采用人胚脑皮质细胞原代培养,感染CVB_3,制备脑炎模型,用双黄连进行抑毒实验。检测指标为培养液肌酸激酶和定量CVB_3测定。结果表明,双黄连实验组对CVB_3感染后的细胞病变有明显抑制作用。结论:双黄连具有抗CVB_3感染的作用。
In order to observe the effects of Shuang Huang Lian, a traditional Chinese medicine, on the pathological changes of human embryonic brain Coxsackie B_3 virus (CVB_3) encephalitis model, primary culture of human embryonic cerebral cortex cells was used to infect CVB_3, an encephalitis model was prepared, and Shuanghuanglian was used for the inhibition experiment. The test indicators were creatine kinase in culture medium and quantitative CVB_3 assay. The results showed that Shuanghuanglian experimental group significantly inhibited the cytopathic effect of CVB_3 infection. Conclusion: Shuanghuanglian has the effect of anti-CVB_3 infection.