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目的 探讨扶正化瘀方对肝星状细胞（HSC）分泌血管内皮生长因子（VEGF）的干预以及对自分泌激活途径的影响。 方法 大鼠灌以扶正化瘀方，制备含药血清；用含药血清添加法观察扶正化瘀方对活化HSC分泌VEGF的影响；以含药血清活化HSC条件培养液温育原代HSC，观察药物对HSC增殖与I型胶原分泌的作用。结果 扶正化瘀方对活化的HSC分泌VEGF的增加有抑制作用；活化的HSC可明显促进静止HSC的增殖与I型胶原的分泌；扶正化瘀方可明显抑制这一促进作用。 结论 扶正化瘀方对HSC的自分泌激活途径有抑制作用，抑制活化的HSC分泌VEGF可能是其作用机制之一。
Objective To investigate the effect of Fuzheng Huayu Decoction on the secretion of vascular endothelial growth factor (VEGF) by hepatic stellate cells (HSC) and its effect on autocrine activation pathway. Methods Rats were treated with Fuzheng Huayu Decoction to prepare drug-containing serum. The effect of Fuzheng Huayu Decoction on the secretion of VEGF in activated HSC was observed by using drug-containing serum. The primary HSCs were incubated with serum containing activated HSC conditioned medium and the drug was observed. HSC proliferation and type I collagen secretion. Results Fuzheng Huayu Decoction can inhibit the increase of VEGF secretion in activated HSCs. Activated HSC can obviously promote the proliferation of type HS and the secretion of type I collagen. Fuzheng Huayu Decoction can obviously inhibit this promotion. Conclusion Fuzheng Huayu Decoction can inhibit the autocrine activation of HSC, and inhibiting the secretion of VEGF by activated HSC may be one of its mechanisms.