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以‘卫士’为砧木,以‘赤峰特选’为接穂进行嫁接,在光照培养箱内对辣椒自根苗(对照)和嫁接苗进行低温(8℃/5℃)弱光(100μmol.m-2.s-1)处理,处理7d后在正常条件(25℃/18℃,550～600μmol.m-2.s-1)下恢复3d,研究低温弱光下辣椒嫁接苗和自根苗电解质渗漏率(EL)、丙二醛(MDA)含量、抗氧化酶活性及根系活力的变化.结果表明:低温弱光胁迫初期,辣椒幼苗叶片与根系的EL、MDA含量和超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性均显著升高,而根系活力大幅降低;1～3d后EL和MDA含量趋于平稳,SOD、POD、APX、GR活性逐渐降低,根系活力呈上升趋势.恢复3d后,嫁接苗EL、MDA含量、抗氧化酶活性及根系活力多达到或超过胁迫前水平(根系的MDA含量较胁迫前略高);而自根苗的EL和MDA含量仍显著高于胁迫前.与自根苗相比,嫁接苗在各处理阶段的EL和MDA含量显著降低,而SOD、POD、APX、GR活性及根系活力明显升高,说明嫁接可有效降低辣椒植株的膜脂过氧化,减轻低温弱光对其细胞膜的伤害.
Taking “guards” as rootstock and grafting with “Chifeng selection”, low temperature (8 ℃ / 5 ℃) low light (100μmol.m-1) 2s-1), and resumed 3d under normal conditions (25 ℃ / 18 ℃, 550 ~ 600μmol.m-2.s-1) after 7 days of treatment to study the electrolyte leakage of grafted and self-rooted pepper under low temperature (EL), malondialdehyde (MDA) content, antioxidant enzyme activities and root activity of the leaves were studied.The results showed that the content of EL, MDA and the activity of superoxide dismutase SOD, POD, APX and GR activities increased significantly, while root activity decreased significantly. After 1 ~ 3d, the content of EL and MDA tended to increase At rest, the activities of SOD, POD, APX and GR decreased gradually, while the activities of roots increased.After 3 days of recovery, the contents of EL, MDA, antioxidant enzyme activity and root activity of grafted seedlings reached or exceeded the levels before stress Slightly higher than that before stress), while EL and MDA contents of self-rooted seedlings were still significantly higher than those before stress.Compared with self-rooted seedling, EL and MDA It was reduced significantly, and SOD, POD, APX, GR activity and root activity was significantly increased, indicating that grafting can effectively reduce the pepper plant membrane lipid peroxidation, reducing its temperature and poor light damage cell membranes.