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文中比较了正常大鼠与矽肺大鼠肺泡巨噬细胞中蛋白质与磷脂的组成,以及酸性磷酸酶活性与耗氧量的差别。根据SDS-PAG电泳的结果看到矽肺大鼠肺泡巨噬细胞的蛋白质区带比正常者多两条,但缺少了一条分子量较小的区带。矽肺大鼠肺泡巨噬细胞中含有大量卵磷脂,说明石英能引起肺表面活性物增多。同时,溶血卵磷脂也比正常者明显地增多,酸性磷酸酶活性与耗氧量均比正常者低,这些都说明石英改变了细胞的组成,破坏了细胞的功能。用克矽平(PVNO)治疗过的大鼠肺泡巨噬细胞的化学组成及酶活性都与正常者近似,证明此药能保护巨噬细胞防止被石英破坏。津_5、山梨醇铝等药物对细胞也表现有不同程度的保护作用。
The composition of protein and phospholipid in alveolar macrophages and the difference between acid phosphatase activity and oxygen consumption in normal and silicotic rats were compared. According to the results of SDS-PAG electrophoresis, the protein bands of alveolar macrophages in silicotic rats were two more than normal, but a small molecular weight band was missing. The alveolar macrophages in silicotic rats contain a large amount of lecithin, indicating that quartz can cause increased pulmonary surfactant. At the same time, lysolecithin was also significantly increased compared with the normal, and the activity of acid phosphatase and oxygen consumption were both lower than those of normal people. All these indicate that quartz changed the composition of cells and destroyed the function of cells. The chemical composition and enzymatic activity of rat alveolar macrophages treated with PVNO were similar to those of normal subjects, demonstrating that this drug can protect macrophages from being destroyed by quartz. Drugs such as argentine _5 and aluminum sorbitol also show different levels of protective effects on cells.