论文部分内容阅读
目的:探讨骨髓间充质干细胞联合螺内酯抑制糖尿病心肌病心肌纤维化的可能机制。方法:建立糖尿病心肌病大鼠模型:SD大鼠随机分为对照组与模型组,对照组普通喂养,模型组通过小剂量链脲佐菌素(STZ)及高糖高脂饲养。通过超声、血流动力学、病理染色等评判模型。将构模成功的模型大鼠随机分为3组予以饲养:PBS组(尾静脉注射PBS),干细胞组(干细胞移植),联合组(干细胞移植及螺内酯灌胃)。通过Western Blot检测各组大鼠14-3-3蛋白的表达。结果:(1)通过血糖、血脂、心脏指数、心脏超声及血流动力学检测,判断糖尿病心肌病模型成功;(2)免疫荧光及流式细胞学检测证实干细胞移植到模型大鼠体内;(3)干细胞组及联合组的心肌纤维化要低于PBS组,而14-3-3蛋白表达较PBS组明显上升(均P<0.05),联合组更为明显。结论:骨髓干细胞通过上调14-3-3蛋白表达抑制糖尿病心肌病的心肌纤维化,和螺内酯有协同作用。
Objective: To investigate the possible mechanism of bone marrow mesenchymal stem cells combined with spironolactone in inhibiting myocardial fibrosis in diabetic cardiomyopathy. Methods: To establish a rat model of diabetic cardiomyopathy: SD rats were randomly divided into control group and model group. The control group was fed normal diet. The model group was fed with low dose of streptozotocin (STZ) and high glucose and high fat. By ultrasound, hemodynamics, pathological staining and other evaluation models. The model rats were randomly divided into three groups: PBS group (tail vein injection PBS), stem cell group (stem cell transplantation), combination group (stem cell transplantation and spironolactone). Western Blot was used to detect the expression of 14-3-3 protein in each group. Results: (1) The success of diabetic cardiomyopathy model was judged by blood glucose, blood lipid, cardiac index, cardiac ultrasound and hemodynamics; (2) Stem cells were transplanted into the model rats by immunofluorescence and flow cytometry; ( 3) Compared with PBS group, the expression of 14-3-3 protein in the stem cell group and the combined group was lower than that in the PBS group (all P <0.05), and the combined group was more obvious. CONCLUSION: Bone marrow stem cells can inhibit myocardial fibrosis of diabetic cardiomyopathy by up-regulating 14-3-3 protein expression, and have synergistic effect with spironolactone.