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本文作者对胚胎发育中表皮内的郎格罕细胞(LC)进行了一系列的研究。根据病史及对胎儿的测量来决定估计胎龄(EGA),EGA在10周或10周以内的为胚胎,10周以上的为胎儿。取35例EGA为45～170天的人胚胎及胎儿下肢皮肤,制成表皮片,用三磷酸腺苷酯酶(ATPase)染色证实在胚胎及胎儿的表皮中有LC存在。EAG至少为45天时LC才出现。用HLA-DR和ATPase单标或双标技术证实:EAG45天时的LC呈现HLA-DR~+,在EAG为50～100天时,HLA-DR~+细胞的密度平均100/mm~2。虽然表皮LC的密度在胚胎与胎儿发育期间,统计学上是相等的,但由于胚胎表皮中的LC较胎儿表皮中的为
The authors conducted a series of studies on the Langerhans cells (LCs) in the epidermis during embryogenesis. Estimated gestational age (EGA) is based on medical history and measurement of the fetus. EGA is an embryo within 10 weeks or less and fetuses over 10 weeks. Twenty-five human embryos and fetal lower extremity skin from 45 to 170 days of EGA were made into epidermal patches, and the presence of LC in the epidermis of embryos and fetuses was confirmed by adenosine triphosphatase (ATPase) staining. LC appears only when EAG is at least 45 days. Using HLA-DR and ATPase single or double-stranded technique, we found that LC showed HLA-DR + at 45 days of EAG, and the average density of HLA-DR + cells was 100 / mm ~ 2 when EAG was 50-100 days. Although the density of cuticle LC is statistically equal during embryonic and fetal development, since the LC in the embryonic epidermis is higher than that in fetal epidermis