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采用Folch液超声提取细雕刻肋海胆性腺中脂肪酸类脂,经氢氧化钾皂化、甲醇-三氟化硼法甲酯化后,以毛细管气相色谱法、气相色谱-质谱联机技术进行了成分分离分析,配合以线性程序升温等当碳长度值结合标准品对照的方法,对样品中的脂肪酸甲酯定性,用峰面积归一化法定量,并且将脂肪酸甲酯的相对质量分数近似认定为脂肪酸的相对质量分数。鉴定了34种脂肪酸,占峰面积的92.5%。样品中不饱和脂肪酸质量分数超过60%,脂肪酸主要成分(质量分数)为:十六碳酸(13.72%),(Z,Z,Z,Z,Z)-5,8,11,14,17-二十碳五烯酸(12.77%)、(Z,Z,Z,Z,Z,Z)-4,7,10,13,16,19-二十二碳六烯酸(10.63%)、E-6-十八碳烯酸(9.45%)、硬脂酸(7.98%)、Z-11-十八碳烯酸(6.43%)。
Folch solution was used to extract fatty acid lipids from the gonads of fine carved sea urchin, which was saponified by potassium hydroxide and methyl esterified with methanol - boron trifluoride. The constituents were separated by capillary gas chromatography and gas chromatography - mass spectrometry , With the linear program temperature equivalent length of carbon combined with the standard method of control, the sample of fatty acid methyl ester qualitative, peak area normalization method to quantify, and the relative mass fraction of fatty acid methyl ester approximately identified as fatty acid Relative mass fraction. 34 fatty acids were identified, accounting for 92.5% of the peak area. The content of unsaturated fatty acid in the sample is more than 60%. The main components of fatty acid are: (16.72%), (Z, Z, Z, Z, Z) -5,8,11,14,17- Eicosapentaenoic acid (12.77%), (Z, Z, Z, Z, Z, Z) -4,7,10,13,16,19- docosahexaenoic acid (10.63%), E Octadecenoic acid (9.45%), stearic acid (7.98%) and Z-11-octadecenoic acid (6.43%).