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目的应用盐酸利多卡因致小鼠中毒惊厥模型,探讨硫酸镁对利多卡因中毒小鼠惊厥和死亡率的影响。方法 210只昆明小鼠,体重18～25g,按照随机数字表随机分为5组:生理盐水对照组、50mg/kg硫酸镁组、100mg/kg硫酸镁组、200mg/kg硫酸镁组、400mg/kg硫酸镁组,每组42只,分别预先腹腔注射4种剂量的硫酸镁或等容积的生理盐水,15min后腹腔注射100mg/kg利多卡因,观察小鼠惊厥潜伏期、持续期、惊厥发生率、死亡时间和死亡率。结果①与生理盐水组相比,硫酸镁可延长利多卡因致惊厥的潜伏期,缩短利多卡因致惊厥的持续时间,延长利多卡因致惊厥的死亡时间。②硫酸镁可以降低利多卡因致惊厥的发生率,200和400mg/kg硫酸镁组与生理盐水组比较,利多卡因致惊厥的发生率差异具有统计学意义(P﹤0.05);可降低利多卡因致惊厥的死亡率,50、100、200mg/kg硫酸镁组与生理盐水比较,利多卡因致惊厥的死亡率差异具有统计学意义(P﹤0.01,P﹤0.05)。③硫酸镁可降低利多卡因致惊厥的强度,随着硫酸镁剂量的增加,利多卡因致惊厥的等级逐渐降低。结论在本实验条件下,硫酸镁可拮抗利多卡因的致惊厥作用,降低利多卡因的毒性。
Objective To investigate the effect of magnesium sulfate on seizure and mortality in mice with lidocaine poisoning by using lidocaine hydrochloride-induced convulsion model in mice. Methods A total of 210 Kunming mice weighing 18-25 g were randomly divided into 5 groups according to a random number table: saline control group, 50 mg / kg magnesium sulfate group, 100 mg / kg magnesium sulfate group, 200 mg / kg magnesium sulfate group, 400 mg / kg magnesium sulfate group, with 42 rats in each group. Four doses of magnesium sulfate or an equal volume of normal saline were injected intraperitoneally, and 100 mg / kg of lidocaine was injected intraperitoneally 15 min later. The incidence of convulsions, the duration of seizures and the incidence of seizures were observed. , Time of death and death rate. Results Compared with the saline group, magnesium sulfate prolonged the incubation period of lidocaine-induced convulsion, shorten the duration of lidocaine-induced convulsion and prolonged the death of lidocaine-induced convulsion. ② magnesium sulfate can reduce the incidence of lidocaine-induced convulsions, 200 and 400mg / kg magnesium sulfate group compared with the saline group, the incidence of lidocaine-induced convulsion was statistically significant (P <0.05); can reduce the profit The mortality rate of caffeine induced convulsion was 50,100,200 mg / kg magnesium sulfate group compared with saline, the difference of the mortality rate of lidocaine-induced convulsion was statistically significant (P <0.01, P <0.05). ③ Magnesium sulfate can reduce the lidocaine-induced convulsions intensity, with increasing dose of magnesium sulfate, lidocaine-induced convulsions gradually reduced the level. Conclusion Under the experimental conditions, magnesium sulfate can antagonize the induced convulsion of lidocaine and reduce the toxicity of lidocaine.