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目的观察“六郁同治”法对动脉粥样硬化易损斑块ApoE-/-模型小鼠血脂及高敏C反应蛋白(hsCRP)表达的影响,探讨其稳定易损斑块的作用机制。方法将6周龄ApoE-/-小鼠高脂喂养24周后随机分为中药高剂量组、中药低剂量组、辛伐他汀组、模型组,治疗12周后检测其血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、hsCRP水平,并观察斑块病理形态。结果光镜下显示造模小鼠主动脉管壁厚薄不均,可见由大量泡沫细胞及胆固醇结晶形成的粥样斑块期病灶,表示造模成功。与模型组比较,中药低剂量组、中药高剂量组、辛伐他汀组血清TC、TG、LDL-C水平明显降低(P<0.05),HDL-C水平明显升高(P<0.05),斑块内脂质成分减少(P<0.01),hsCRP表达水平降低(P<0.01)。结论 “六郁同治”法可稳定易损斑块,延缓动脉粥样硬化进程,其作用机制可能与抑制血脂代谢及炎性因子hsCRP表达有关。
Objective To observe the effect of “Liu Yu Tongzhi” method on the expression of lipids and hsCRP in ApoE - / - mice with vulnerable atherosclerotic plaques and to explore the mechanism of stabilizing vulnerable plaque. Methods Six weeks of ApoE - / - mice were fed with high fat diet for 24 weeks and then randomly divided into high dose Chinese medicine group, low dose Chinese medicine group, simvastatin group and model group. After 12 weeks of treatment, serum total cholesterol (TC) , Triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and hsCRP were measured. The plaque pathology was also observed. Results The light microscope showed that the thickness of the aortic wall of the model mice was uneven. It showed that atherosclerotic plaque stage was formed by a large number of foam cells and cholesterol crystals, indicating successful modeling. Compared with the model group, the levels of TC, TG and LDL-C in the low-dose group, high-dose group and simvastatin group were significantly decreased (P <0.05), the HDL-C level was significantly increased Lipid composition decreased (P <0.01), hsCRP expression decreased (P <0.01). Conclusion “Six Yu Tongzhi ” method can stabilize vulnerable plaque, slow the process of atherosclerosis, the mechanism may be related to the inhibition of blood lipid metabolism and the expression of inflammatory factor hsCRP.