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目的:建立测定人血浆中多沙普仑浓度的HPLC法。方法:血浆样品经液-液萃取处理后进行测定。色谱柱为Dia-monsil C_(18)柱(200mm×4.6mm,5μm);柱温为室温;流动相为磷酸二氢铵缓冲液(0.01 mol·L~(-1),pH4.5)-甲醇-乙腈(56:30:14);流速为1.3ml·min~(-1);紫外检测波长为214nm;内标为非那西丁。结果:多沙普仑血药浓度在0.05～5μg·ml~(-1)范围内线性关系良好(r=0.999 0);提取回收率为60.6%～64.0%;日内RSD小于6.5%,日间RSD小于13.4%。结论:此方法操作简便,灵敏,准确,适用于多沙普仑的临床药物动力学研究。
Objective: To establish an HPLC method for the determination of doxapram in human plasma. Methods: Plasma samples were determined by liquid-liquid extraction. The column was Dia-monsil C 18 column (200 mm × 4.6 mm, 5 μm). The column temperature was at room temperature. The mobile phase was ammonium dihydrogen phosphate buffer (0.01 mol·L -1, pH 4.5) Methanol-acetonitrile (56:30:14); flow rate of 1.3ml · min -1; UV detection wavelength of 214nm; internal standard of phenacetin. Results: The plasma concentration of doxapram had good linearity (r = 0.999 0) in the range of 0.05-5 μg · ml -1; the recovery was 60.6% -64.0%; the intraday RSD was less than 6.5% RSD is less than 13.4%. Conclusion: This method is simple, sensitive, accurate and suitable for clinical pharmacokinetics of doxapram.