论文部分内容阅读
采用纤维素复合酶直接酶解(Direct enzymolysis,DE)、1% H2SO4预处理后酶解(Enzymolysis after 1% H2SO4 pretreatment,ACE)和1%NaOH预处理后酶解(Enzymolysis after 1% NaOH pretreatment,ALE)3种处理方法对2种生态型柳枝稷Alamo(低地型)和Cave-in-Rock(高地型)茎秆进行降解效率的研究,并对其细胞壁组成特点进行分析。结果表明:1)DE处理下,Alamo茎秆总产糖效率、六碳糖产糖效率和纤维素降解效率分别显著高于Cave-in-Rock 24.94%、40.52%和24.68%(P<0.05或0.01);ACE处理下,Alamo茎秆预处理产糖效率、酶解产糖效率和总产糖效率分别显著高于Cave-in-Rock 17.13%、25.69%和16.87%,五碳糖产糖效率、六碳糖产糖效率和纤维素降解效率分别显著高于Cave-in-Rock 9.71%、38.93%和21.86%;ALE处理下,Alamo茎秆酶解产糖效率和总产糖效率分别显著高于Cave-in-Rock 16.69%和13.48%,五碳糖产糖效率、六碳糖产糖效率和纤维素降解效率分别显著高于Cave-in-Rock 13.07%、17.46%和6.59%;2)Alamo茎秆细胞壁中纤维素含量显著高于Cavein-Rock 11.31%,纤维素结晶度和聚合度分别显著低10.09%和40.54%;半纤维素中阿拉伯糖(Ara)含量显著高于Cave-in-Rock 5.28%,而木糖(Xyl)含量显著低于Cave-in-Rock 1.37%,阿拉伯糖替代程度(Ara/Xyl)显著高于Cave-in-Rock 6.74%;木质素单体紫丁香基(S)含量显著高于Cave-in-Rock 16.40%。较高的纤维素含量、较低的纤维素结晶度和聚合度是Alamo茎秆高产糖效率的直接原因,推测阿拉伯糖的替代程度对纤维素结晶度和聚合度的影响以及较高含量的木质素单体S可能是Alamo茎秆高降解效率的深层次原因。
Enzymolysis after 1% NaOH pretreatment (ACE) and 1% NaOH pretreatment were used to study the effects of Enzymolysis after 1% NaOH pretreatment, ALE) were used to study the degradation efficiency of two ecotypes of Alamo (Lowland) and Cave-in-Rock (Highland) stems, and their cell wall composition characteristics were analyzed. The results showed that: 1) DE treatment, the Alamo stalk total sugar production efficiency, six sugar production efficiency and cellulose degradation efficiency were significantly higher than Cave-in-Rock 24.94%, 40.52% and 24.68% (P <0.05 or 0.01). Under the treatment of ACE, the sugar-producing efficiency, enzymolysis-producing sugar yield and total sugar yield of Alamo stalk pretreatment were significantly higher than that of Cave-in-Rock 17.13%, 25.69% and 16.87%, respectively. , The yield of hexose sugar and the rate of cellulose degradation were significantly higher than those of Cave-in-Rock 9.71%, 38.93% and 21.86%, respectively. Under the treatment of ALE, the sugar yield and total sugar yield of Alamo stalk were significantly higher At 16.69% and 13.48% of Cave-in-Rock, the sugar-producing efficiency, sugar-producing efficiency and cellulose-degrading efficiency of pentavalent sugar were significantly higher than those of Cave-in-Rock by 13.07%, 17.46% and 6.59% The cellulose content of Alamo stalk cell wall was significantly higher than that of Cavein-Rock 11.31%, cellulose crystallinity and degree of polymerization were significantly lower 10.09% and 40.54%, respectively; the content of arabinose in hemicellulose was significantly higher than that of Cave-in- Rock 5.28%, while Xyl content was significantly lower than Cave-in-Rock 1.37% and Ara / Xyl was significantly higher than Cave-in-Rock 6.74%. Lignin monomer Syringyl (S) were significantly higher than Cave-in-Rock 16.40%. Higher cellulose content, lower cellulose crystallinity and degree of polymerization are the direct causes of the high yield of sugar in Alamo stalk. It is estimated that the degree of substitution of arabinose affects the crystallinity and degree of polymerization of cellulose and the higher content of wood Monomeric S may be the underlying cause of the high efficiency of Alamo stalk degradation.