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通过许多免疫酶试验证明,辣根过氧化物酶(HRP)标记抗体是有效的。制备酶标抗体常用二步法,方法简单,结合物主要由抗体和酶所组成,两者的比率为1:1,但大部分反应产物是游离的抗体。因为未标记抗体能和标记抗体竞争抗原的位点,所以未标记抗体明显降低免疫酶试验的敏感性。标记的和未标记的抗体在分子量和电荷上仅有微小差异,要将二者分开是困难的。
Many immune enzyme tests have shown that horseradish peroxidase (HRP) -labeled antibodies are effective. Preparation of enzyme-labeled antibody commonly used two-step method, the method is simple, the conjugate is mainly composed of antibodies and enzymes, the ratio between the two is 1: 1, but most of the reaction products are free antibodies. Unlabeled antibodies significantly reduce the sensitivity of the immunoenzyme assay because unlabeled antibodies compete with the labeled antibody for the site of the antigen. Both labeled and unlabeled antibodies have only minor differences in molecular weight and charge, and separation of the two is difficult.