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探索利用活血生物效价检测方法和化学指纹图谱方法评价大黄的品质。采用血小板聚集仪测定不同大黄药材体外抗血小板聚集率,根据简化概率单位法计算生物效价;采用超高效液相色谱仪建立不同大黄药材的指纹图谱;利用SPSS 22.0软件的双变量(bivariate)分析方法进行谱效相关分析,最后用化学单体进行了验证。活血效价测定结果显示掌叶大黄的活血效价普遍高于唐古特大黄和药用大黄;酒大黄的活血效价高于生大黄,大黄炭活血效价显著降低。指纹图谱中23个共有色谱峰,指认了其中10个成分。谱效相关分析得到3个相关性较好的化学成分,包括大黄酸-8-O-β-D-葡萄糖苷、大黄素-8-O-β-D-葡萄糖苷和大黄酸,活血效价验证结果表明三者均具有较强的抑制血小板聚集的作用。本文建立的基于测定血小板聚集率模型的大黄活血生物效价检测方法对于大黄药材,尤其是酒大黄炮制过程中的质量评控具有一定的参考价值,同时初步证明大黄中活血化瘀药效物质为大黄酸等蒽醌类衍生物。
To explore the use of biological activity of blood test methods and chemical fingerprints method to evaluate the quality of rhubarb. Platelet aggregation was used to determine the antiplatelet aggregation rate of different rhubarb herbs in vitro. Bioequivalence was calculated according to the simplified probability unit method. Fingerprints of different rhubarb herbs were established by ultra performance liquid chromatography. Bivariate analysis was performed using SPSS 22.0 software. Method for spectral correlation analysis, and finally verified with chemical monomers. The results of determination of activated blood titer showed that the potency of activating blood of Rheum palmatum was generally higher than that of Tangut rhubarb and medicinal rhubarb; the blood activating potency of rhubarb was higher than that of rhubarb, and the blood titer of rhubarb was significantly decreased. The 23 common peaks in the fingerprinting fingerprint identified 10 of them. Spectral correlation analysis showed that three chemical components with good correlations, including rhein-8-O-β-D-glucoside, emodin-8-O-β-D-glucoside and rhein, Validation results show that all three have a strong inhibitory effect on platelet aggregation. The method for detecting the bioactivity of rhubarb and activated blood based on the platelet aggregation rate model established in this paper has certain reference value for the quality control of Rhubarb, especially rhubarb, and at the same time, it proves that the effective substances of rhubarb Rhein and other anthraquinone derivatives.