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以平菇为产纤溶酶菌种,采用单因素实验,确定该菌株产纤溶酶的最适发酵培养基为:葡萄糖2%,豆浆15%,KH2PO40.3%,MgSO40.15%;最适培养条件为:初始pH为自然,接种量为直径1cm的菌片1片,培养基装液量80mL(250mL三角瓶),培养温度25℃,摇床转速150r/min,发酵周期6d。在此条件下,发酵液纤溶酶活力(相当于尿激酶活力单位)稳定达到5.20IU/mL。
Taking the Pleurotus ostreatus as fibrinolytic enzyme, single-factor experiments were conducted to determine the optimal fermentation medium for producing fibrinolytic enzyme of this strain: glucose 2%, soybean milk 15%, KH2PO40.3%, MgSO40.15% The suitable culture conditions were as follows: the initial pH was natural, inoculum was 1 piece of 1cm in diameter, the medium was filled with 80mL (250mL flask), the culture temperature was 25 ℃, the speed of shaking was 150r / min and the fermentation period was 6 days. Under these conditions, the activity of fibrinolytic enzyme (equivalent to urokinase activity unit) of fermentation broth reached 5.20IU / mL.